摘要
本研究使用具有廣用性改質功能的環氧型兩性離子共聚高分子PGMA-co-PSBMA（poly(glycidyl methacrylate-co-sulfobetaine methacrylate)）對丁腈橡膠（nitrile-butadiene rubber, NBR）進行生物相容性改質。 藉由化學改質法中最具經濟效益的表面接枝法（grafting to）以浸泡塗佈（dip-coating）的方式讓功能端接枝上所需的改質的丁腈橡膠表面形成”改質NBR” （m-NBR）。透過最佳化製程: 塗佈溶液為3 mg/ml （PGMA-co-PSBMA/H2O）、溶液溫度為80°C、反應時間控制為3 hr，可得到最佳之表面接枝密度。 經過油與水接觸角實驗得知，疏水親油的丁腈橡膠經由處理後能變成親水疏油的改質丁腈橡膠。 經過ATR-FTIR和XPS的表面官能基檢測確定m-NBR表面擁有C=O（1660-1770 cm-1 ）及更強烈的O-H（3300-3800 cm-1）訊號，證明高分子確實有接枝上NBR。 接著針對m-NBR進行生物性質檢測，結果發現，塗佈上PGMA-co-PSBMA的m-NBR可以抵抗廣泛的蛋白質吸附，最佳能夠降低87%的吸附量。 在抵抗血球的貼附（紅血球RBC (red blood cells）、血小板PRP (platelet-rich plasma）及貧血小板的血漿PPP (platelet-poor plasma)）最佳能夠降低90%的貼附量。 最後在抵抗細菌的貼附（大腸桿菌 E.coli）上，最佳能夠降低70%的細菌貼附量。以上的結果證實，PGMA-co-PSBMA塗布過後的m-NBR可達到理想的抗生物沾黏效果。
關鍵詞：丁腈橡膠、兩性離子、抗生物沾黏、表面改質
Abstract
In this study, nitrile-butadiene rubber (NBR) was modified by epoxylated zwitterionic copolymer polymer PGMA-co-PSBMA (poly(Glycidyl methacrylate-co-sulfobetaine methacrylate)) to improve its biocompatibility. By means of the most economical surface grafting method in the chemical modification method (grafting to), the functional end was grafted on the surface of the NBR through the dip-coating method and forming a PGMA-co-PSBMA modified surface (m-NBR). Through the optimization process to achieve the best graft density, the concentration of coating solution was 3 mg/ml (PGMA-co-PSBMA/H2O), the controlled coating temperature is 80°C, and the reaction time was controlled for 3 hours. The oil-water contact angle experiment showed that the hydrophobic and oleophilic nitrile rubber can be transformed into a hydrophilic and oleophobic modified nitrile rubber after treatment. The surface functional group detection of ATR-FTIR confirmed that the surface of m-NBR has C=O (1660-1770 cm-1 ) and more intense O-H (3300-3800 cm-1), which proves that the polymer is indeed grafted with NBR. The biological properties of m-NBR were tested, and it was found that m-NBR can resist the adsorption of a wide range of proteins and can reduce the adsorption capacity by 87%. The reduction of blood cell attachments (erythrocyte RBC (red blood cells), platelet PRP (platelet-rich plasma) and platelet-poor plasma PPP (platelet-poor plasma) ) is about 90 %. Finally, the adhesion against bacteria (Escherichia coli E.coli) can reduce the amount of adhesion by 70 %. The above data proved that the m-NBR coated with PGMA-co-PSBMA can achieve the ideal anti-biofouling effect.
Keywords: Nitrile-butadiene RubberAbstract