摘要
本研究利用離心力法製備生醫微米纖維，取代以往採用靜電紡絲法，可快速生產低成本之微米纖維並應用於敷料等醫療製品。本研究使用自行開發之專利離心微流道裝置，以轉動馬達作為核心，並帶動圓形壓克力微流道旋轉，微流道以放射狀自圓心向周邊延伸，孔徑為700 μm，長度為1.3 cm、離心轉速為7500 rpm-15000 rpm，將明膠溶液注入微流道利用離心轉盤噴絲固化，藉此產生品質良好及高軸向性之纖維。本研究製備20 wt%~40 wt%的明膠溶液作為測試原料，討論溶液濃度、溶液量及離心轉數等參數對於纖維型態及尺寸之影響。實驗利用光學顯微鏡觀察纖維絲之尺寸及型態，並利用紫外-可見光光譜儀進行樣品絲之收集效率及透明度分析，分別利用摩擦係數儀及材料試驗機量測纖維表面粗糙度及纖維強度。研究結果顯示，35 wt%及40 wt%的明膠溶液能夠產生較佳的纖維，前者可產生直徑為20 μm~100 μm之微米纖維，後者可產生直徑為10 μm~20 μm之微米纖維。此外，離心轉速為11000 rpm時可產生最均質的纖維絲。
關鍵詞：離心力法、生醫微米纖維、微流道、明膠、纖維強度
Abstract
In this study, we successfully fabricated the microfibers for medical applications by using the centrifugal force method. We used a proprietary centrifugal microchannel device equipped with a rotary motor to drive circular acrylic microchannels. The aperture and length of the micro-channel were 700 μm and 1.3 cm, respectively, and the device was centrifuged at 7,500 rpm to 11,000 rpm. The gelatin solution was injected into the micro channel and then solidified with a centrifugal spinneret to produce fibers with good quality and high axiality. A series gelatin solution from 20 wt% to 40 wt% were prepared as test materials. The effects of solution concentration, solution volume, and centrifugal rotation speed on the optical and mechanical properties of the fibers were investigated. Additionally, the size and shape of the microfibers were observed by using an optical microscope. The collection efficiency and transparency of the microfibers were analyzed by using a spectrometer. The fracture strength and surface roughness of the gelatin microfibers were determined by using a tensile testing machine and a friction coefficient meter, respectively. The results showed that the high quality microfibers with diameters ranging from 20 μm to 100 μm and 10 μm to 20μm could be obtained from gelatin solutions with concentrations of 35 wt% and 40 wt%, respectively. Furthermore, it was found that the most homogeneous microfibers could be formed at a centrifugal rotation speed of 11000 rpm.
Keywords: Centrifugal force method, Medical microfiber, Micro-channel, Gelatin, Fiber strength